Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 2020 , Vol 11, Issue 4
The Effect of Short-Chain Fatty Acids On Epithelial Repair In Experimental Colitis Created With Trinitrobenzenesulfonic Acid In Rats
Müjdat Kara1,H. Ahmet Tezel2
1Acıbadem Altunizade Hastanesi, Endokrinoloji ve Metabolizma Hastalıkları Bilim Dalı, İstanbul, Türkiye
2Trakya Üniversitesi Tıp Fakültesi , Gastroenteroloji Bilim Dalı, Edirne, Türkiye
DOI : 10.31067/0.2020.280 Objective: The purpose of this study is to discuss short-chain fatty acids" (SCFA) role in ulcerative colitis (UC) treatment by using rats which have experimental colitis created with trinitrobenzenesulfonic acid (TNBS).

Method: In this study, the experimental research method was adopted. The study group consisted of 30 female Wistar rats which have 150±30 gr. average weights, Rats were randomly divided into 3 groups with 10 rats in each group. The groups were randomly assigned as two experimental groups and one control group. The first experimental group was administered SCFA intracolonically 2 times a day from the 3rd day, and the second experimental group was administered SCFA 2 times a day from the first day. The control group was given saline 2 times a day from the 3rd day. On day 6, the rats were decapitated. MPO activity evaluation and macroscopic and microscopic evaluation of the colon mucosa was done.

Results: There was a statistically significant difference between the colon macroscopy results of the groups 1-2, 2-3, 1-3 (p<0.05, p<0.01; p<0.01). There was not a statistically significant difference between the colon MPOs of Group 1 and 2 (p=0.139). Groups 1-3 and 2-3 colon MPOs differed from each other significantly (p<0.05; p<0.01).

Discussion: According to the results of this study, colon macroscopic results and MPO activities in Group 2 were significantly successful compared to the first and third groups. This finding suggests that SCFA may be an alternative option in the treatment of UC. Keywords : Short-chain fatty acids. ulcerative colitis, trinitrobenzenesulfonic acid